Spectroscopic diagnosis of CFS
Visible and near-infrared spectroscopy in CFS serum samples
Abstract
To investigate visible and near-infrared (Vis-NIR) spectroscopy enabling chronic fatigue syndrome (CFS) diagnosis, we subjected sera from CFS patients as well as healthy donors to Vis-NIR spectroscopy. Vis-NIR spectra in the 600-1100 nm region for sera from 77 CFS patients and 71 healthy donors were subjected to principal component analysis (PCA) and soft independent modeling of class analogy (SIMCA) to develop multivariate models to discriminate between CFS patients and healthy donors. The model was further assessed by the prediction of 99 masked other determinations (54 in the healthy group and 45 in the CFS patient group). The PCA model predicted successful discrimination of the masked samples. The SIMCA model predicted 54 of 54 (100%) healthy donors and 42 of 45 (93.3%) CFS patients of Vis-NIR spectra from masked serum samples correctly. These results suggest that Vis-NIR spectroscopy for sera combined with chemometrics analysis could provide a promising tool to objectively diagnose CFS.
Biochemical and Biophysical Research Communications Volume 345, Issue 4 , 14 July 2006, Pages 1513-1516
Also cited at:
Spectroscopic Blood Test as Tool for Diagnosing CFS subset, July 10, 2006
Infrared Spectroscopy, July 1, 2006
NIR background
Near Infrared Spectroscopy (NIRS) has been used to measure regional skeletal muscle deoxygenation [1-4] by presenting a balance between oxygen delivery and demand in muscle tissue. NIRS is beneficial since it is non-invasive and does not restrict ongoing activities at sports events such as skiing [5] and skating [6]. Further, it is used to measure local muscular tissue circulation, namely blood flow [7] and oxygen consumption (O2) [8]. The validity of these measurements and their relationship to global metabolism has been well established [9,10]. Thus, NIRS technology contributes to the understanding of regional muscular metabolism and O2 transport systems.
Dynamic Medicine 2006, 5:5 doi:10.1186/1476-5918-5-5
More about spectroscopic imaging technology, a new field.
By Ralph Weissleder, director of the Center for Molecular Imaging Research at Massachusetts General Hospital and Harvard Medical School
A Wikipedia summary:
"Infrared radiation spectroscopy is the study of the composition of (usually) organic compounds, finding out a compound's structure and composition based on the percentage transmittance of IR radiation through a sample. Different frequencies are absorbed by different stretches and bends in the molecular bonds occurring inside the sample."
"Near infrared spectroscopy (NIRS) is a spectroscopic method utilising the near infra-red region of the electromagnetic spectrum (from about 800nm to 2500nm). Typical applications include pharmaceutical, medical diagnostics (including blood sugar and oximetry), food and agrochemical quality control, as well as combustion research."
